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KMID : 0545120020120050813
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 5 p.813 ~ p.818
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Synergistic Effect of Lipopolysaccharide and Interferon-βon the Expression of Chemokine Mig mRNA
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LEE, MOON-SOOK
KIM, SUNG-KWANG/KIM, HEE-SUN
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Abstract
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Expression if monokine induced by IFN-γ(Mig) mRNA is well-known to strictly depend on Interferon-γ(IFN-γ). Lipopolysaccharide (LPS) alone is weakly effective on Mig mRNA expression in mouse peritoneal macrophages. This study was undertaken to investigate the synergistic effect of LPS and IFN-β on chemokine Mig gene expression in mouse peritoneal macrophages. Although IFN-β alone was minimally effective, LPS plus IFN-β synergized to produce a high level of Mig mRNA. The synergistic effect of LPS and IFN-β (LPS/ IFN-β_ on Mig mRNA expression was strain-specific. The most effective synergistic effect of LPS/ IFN-β on the mRNA expression was found in simultaneous stimulation of LPS/ IFN-β. This synergy was modulated at the level of the gene transcription and was not dependent on a new protein synthesis. Synergistic effect of LPS/ IFN-β also required the activation of NF-κB. Accordingly, these data suggest that LPS/ IFN-β synergizes the expression of Mig mRNA through a process that depends on a pretranscriptional level and/or coincident Mig mRNA transcription.
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KEYWORD
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